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. 2012 Apr 26;7(4):e35645. doi: 10.1371/journal.pone.0035645

Figure 4. Neriifolin-induced DA neuronal death is apoptotic and requires p53.

Figure 4

(A–D′″) Low (A–D) and high (A′–D′″) magnification views of VFB DA neurons in control (A-A′″) vs Neriifolin-treated embryos (B-B′″), and sympathetic (Sym) NA neurons in control (C-C′″) vs Neriifolin-treated embryos (D-D′″). Ventral views of 60 hpf embryos were shown. Neriifolin treatment was carried out from 24 hpf to 60 hpf. (E) Injection of the p53-MO into embryos at one-cell stage protected DA neurons from cell death induced by Neriifolin. Data are the averages ± SEM from 6 embryos in a single experiment that was repeated twice with similar results.