Table 1. Beta-cell proliferation and number of total cells evaluated in sorted human beta-cells and non-sorted dispersed islet cells cultured on 3 different matrices in control medium or with Liraglutide and/or conditioned medium (n = 7).
Sorted beta-cells | Non-sorted cells | |||||
Pooled treatmentsa | Control | Lira | CM | CM+Lira | ||
% BrdU+/Ins+ | BCEC | 0.034±0.019 | 0.017±0.008 | 0.082±0.034 * | 0.042±0.014 | 0.027±0.014 |
804 G | 0.015±0.004 | 0.032±0.026 | 0.011±0.005 | 0.049±0.029 | 0.186±0.088 | |
HTB9 | 0.097±0.082 | 0.028±0.025 | 0.047±0.034 | 0.097±0.040 | 0.035±0.024 | |
Sorted beta-cells | Non-sorted cells | |||||
Number of cells counted | Total cells | 85'412 | 525'001 | |||
Beta cells | 84'283 | 232'826 | ||||
BrdU+/Ins+ | 22 | 104 | ||||
% BrdU+/Ins+ | Pooled matrices and treatments | 0.051±0.020 | 0.055±0.011 |
For each matrix, the numbers of proliferating beta-cells were pooled from dishes treated with control media ±Liraglutide or condit.media ± Liraglutide.
represents p<0.05 as tested by Mann-Whitney.
CM, conditioned medium; Lira, Liraglutide; BCEC, bovine corneal endothelial cell; 804 G, rat bladder carcinoma; HTB9, human bladder carcinoma.