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. 2012 Apr 26;7(4):e35755. doi: 10.1371/journal.pone.0035755

Figure 4. Lj-HMGB1 mRNA expression is significantly upregulated in gill tissue after treatment with LPS or ConA.

Figure 4

The lamprey HMGB1 mRNA levels were determined using real-time quantitative RT-PCR in various lamprey tissues. Total RNA was extracted from the gills, intestines, heart, lymphocyte-like cells and kidneys of lampreys after stimulation with LPS or ConA. Lamprey GAPDH served as an internal control to calibrate the cDNA template for all of the samples, and PBS served as a negative treatment control. The significant differences (p<0.05) in HMGB1 expression between the challenged groups and the control group are indicated with asterisks.