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. 2012 Apr 26;7(4):e35740. doi: 10.1371/journal.pone.0035740

Figure 9. PAI-1 influences the pro-survival Akt pathway in human endothelial cells by PTEN inactivation.

Figure 9

Representative western blots of phospho-Akt, total Akt, phospho-PTEN, total PTEN, phospho-p38MAPK, p65/RelA, phospho-ERK1/2, phospho-PDK-1, Bcl-2 and Bcl-XL in HUVECs transfected or not with siRNA PAI-1 24 hours after 20 Gy (A). Representative western blots of phospho and total Akt in HUVECs that stably overexpress PAI-1. Akt activation is reduced in human ECs that overexpress PAI-1 (B). Silencing efficiency determined by western blot in HUVECs transfected with siRNA PTEN or siRNA PDK-1 (C). Representative images of HUVECs knocked-down for PTEN, PDK-1, PAI-1, PTEN/PAI-1, or PDK-1/PAI-1 24 hours after 20 Gy (D). Representative western blots of phospho-Akt, total Akt, p65/RelA, phospho-ERK1/2, PTEN, BCL-2 and BCL-XL in HUVECs transfected with siRNA PTEN, PDK-1 and/or siRNA PAI-1 24 hours after 20 Gy(E) .