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. 2012 Apr 26;7(4):e35893. doi: 10.1371/journal.pone.0035893

Table 2. Mouse immunization protocols used in this study.

Protocol Immunization route Dosing amount Immunization schedule Adjuvant Blooding Spleen isolation Results
1 Subcutaneous 8 ng per dose Four times at two-week intervals None Ten days after 3rd and 4th immunizations Figure 5
Figure S4
2 Subcutaneous 1000 ng per dose Three times at two-week intervals Aluminum hydroxide (200 µg per dose) Ten days after 3rd immunization Figure 6
Figure S3
3 Subcutaneous 1000 ng per dose Two times at a two-week interval Aluminum hydroxide (200 µg per dose) Ten days after 2nd immunization Figure 7