Table 2. Mouse immunization protocols used in this study.
Protocol | Immunization route | Dosing amount | Immunization schedule | Adjuvant | Blooding | Spleen isolation | Results |
1 | Subcutaneous | 8 ng per dose | Four times at two-week intervals | None | Ten days after 3rd and 4th immunizations | — | Figure 5 |
Figure S4 | |||||||
2 | Subcutaneous | 1000 ng per dose | Three times at two-week intervals | Aluminum hydroxide (200 µg per dose) | Ten days after 3rd immunization | — | Figure 6 |
Figure S3 | |||||||
3 | Subcutaneous | 1000 ng per dose | Two times at a two-week interval | Aluminum hydroxide (200 µg per dose) | — | Ten days after 2nd immunization | Figure 7 |