Figure 1. Patterns of laminin-1 produced using LAPAP.

(a) The platform consists of a 473 nm diode-pumped solid-state laser, a lens and a 3-axis motorized stage to produce the desired patterns of biotin-4-fluorescein. (b) Large gradients (400 µm×600 µm) of laminin-1 were fabricated by sequentially incubating streptavidin-DyLight549, biotinylated goat anti-rabbit IgG (Fc specific), rabbit anti-laminin-1 and laminin-1. This particular pattern was imaged using chicken anti-laminin-1 and Cy5 goat anti-chicken IgG to reveal the presence of laminin-1. The red rectangle delimits the section of the patterns considered for the RGC-5 guidance assay. (c) x-direction mean profile of 25 gradients. The first quartile (on the left) of the pattern was used as a high-slope gradient while the last three quartiles (on the right) were used as a low-slope gradient.