Figure 5. Effect of varying the restriction angle used to define whether an extending neurite was attracted or repusled by the gradient.

(a) Histograms of the most distal end-point position for different restriction angle. (b) Ratio of attracted over repulsed neurites shows how this arbitrary parameter can greatly influence the outcome of the analysis. (Circles = Laminin, Squares = Control 1, Diamonds = Control 2). This analysis demonstrates that high-content screens of neurite guidance are strongly dependent on choice of critical user-defined parameters. Data used from high-slope gradients, three hours after differentiation.