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. 2012 Apr 26;7(4):e36204. doi: 10.1371/journal.pone.0036204

Figure 1. Lethal symptoms observed in hybrid seedlings from crosses between N. occidentalis and N. tabacum.

Figure 1

(A–D) Hybrid seedlings from the cross N. occidentalis ♀×N. tabacum ‘Red Russian’ ♂ at 24°C. Although hybrid seedlings at 3 days after germination (DAG) exhibited apparently normal growth, root growth nearly stopped (A). The color of cotyledons and hypocotyl faded beginning from 8 DAG (B), continued to advance at 10 DAG (C), with the seedlings turning completely brown and dying by 30 DAG (D). (E–G) N. occidentalis grown at 24°C. N. occidentalis grew normally and did not show lethal symptoms at 3 (E), 10 (F), or 30 DAG (G). (H–J) ‘Red Russian’ grown at 24°C. ‘Red Russian’ also grew normally and did not display lethal symptoms at 3 (H), 10 (I), or 30 DAG (J). (K, L) Hybrid seedlings from the cross (N. tabacum Haplo-Q×N. tabacum ‘Samsun NN’) ♀×N. occidentalis ♂. Hybrid seedlings possessing (K) and lacking (L) the Q chromosome died after being transferred from 34°C (left panels) to 24°C (right panels). Scale bars = 1 mm (A–J) and 5 mm (K, L).