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Network of potential interactions among tRNAs (cloverleaf) and synthetases (“C-shape”). (A) Hypothetical wild-type case having three cognate pairs. In the typical cell, about 1,200 potential interactions are possible. (B) The increase in network complexity that arises when a new cognate pair is introduced. Colored arrows indicate cognate interactions, and dashed arrows indicate interactions that would result in amino acid misincorporation.
