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. 2012 Apr 27;7(4):e36135. doi: 10.1371/journal.pone.0036135

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Muraoka et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Ultrastructure of photoreceptors in 4-week-old WT rabbits. The inner (IS) and outer segments (OS) of the photoreceptors were regular and dense. There are no vesicles in the extracellular spaces (*). (C–E) Ultrastructure of the photoreceptors in the 4-week-old RP rabbits. The IS and OS were less organized than those in the WT rabbits. In the magnified image (D), the RP rabbit retina showing many small vesicles (arrowheads) accumulated in the extracellular spaces (indicated with * in panel C). The vesicles appeared to be cleaved from the IS into the extracellular space around the photoreceptors (arrows in panel E). (F) Ultrastructural immunohistochemistry by using an anti-rhodopsin antibody. The small vesicles (disintermediated arrowheads) in the extracellular spaces around the photoreceptors exhibit black dots indicating the presence of rhodopsin.