Figure 3. Role of Cx43 in gap-mediated Ca²⁺ transients in NRVMs.

(A) NRVMs were infected with adenovirus vector or adenovirus carrying Cx43 full sequences (wt-Cx43) or Cx43 siRNA (m.o.i. = 15) for 48 h. The expression of Cx43 in NRVMs was determined by Western blot, which was normalized by the level of GAPDH. ** denotes P<0.01 vs. control. (B) Typical traces represent spontaneous Ca²⁺ transients in single and monolayer NRVMs with Cx43 overexpression or knockdown as indicated, and (C) Their statistical data of the transient frequency and amplitude were obtained from 5–6 independent experiments. (D) The statistical data of the dose-dependent effect of Cx43 deficiency on Ca²⁺ transients in single cells were obtained from 7–9 independent experiments. ** represents P<0.01 vs. scramble (Scram) for each panel.