Figure 7. Recovery effect of inositol 1,4,5-trisphosphate on gap inhibitor-induced Ca²⁺ transient inhibition in monolayer NRVMs.

(A) Images and traces represent spontaneous global Ca²⁺ oscillations in monolayer cells prior to and 1 or 30 min after heptanol (1 mM) or Gap 27 (300 µM), and then phenylephrine (PE 20 µM, 5 min) or IP₃/BM (20 µM, 6 min) treatments. (B, C) The summarized data indicate the recovery effect of IP₃ analogues on Gap 27-inhibited Ca²⁺ transients and LY uptake. **P<0.01 vs. control; ^## P<0.01 vs. Gap 27 alone, from 10–18 independent determinations for each bar.