Figure 8. Pathways involved in the Cx43-associated mediation of Ca²⁺ activities in single and monolayer NRVMs.

(A) The summarized data of the effects of nifedipine (Nif 1 µM, 10 min), ryanodine (Rya 100 µM, 10 min) and xestospongin C (XeC 10 µM, 20 min) on the LY uptake and intracellular Ca²⁺ frequency in NRVMs. (B) Typical traces represent the spontaneous Ca²⁺ transients in single and monolayer NRVMs prior to (Con) and after 2-APB (2 µM) for 10 min treatment. (C, D) The statistical data of the concentration-dependent effects of 2-APB on LY loading and Ca²⁺ transient frequency in single and confluent NRVMs as indicated were obtained from 8–12 determinations for each bar. **P<0.01 vs. DMSO in each panel; ^## P<0.01 vs. DMSO treated single cells.