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. 2012 Apr 27;7(4):e36338. doi: 10.1371/journal.pone.0036338

Figure 3. cuf2 + gene expression is Mei4-dependent.

Figure 3

pat1-114/pat1-114 (mei4+/mei4+) and pat1-114/pat1-114 mei4Δ/mei4Δ strains were pre-synchronized by nitrogen starvation at 25°C, and then were induced to undergo synchronous meiosis at 34°C. At the indicated time points, the cuf2+ and act1+ (internal control) mRNA levels were analyzed in both the control strain (mei4+/mei4+) and the isogenic strain lacking the mei4+ alleles. To validate the absence of the cuf2+ transcript during mitosis, total RNA was probed for the presence of cuf2+ mRNA in vegetative cells incubated at 25°C.