Figure 4. Analysis of Cuf2-GFP localization during both meiosis and sporulation.

The Cuf2-GFP fluorescence signal (center left) was observed at different stages of meiosis following the azygotic meiotic induction of a h⁺/h⁻ cuf2Δ/cuf2Δ cuf2⁺-GFP/cuf2⁺-GFP strain. Once induced, azygotic meiotic cells were differentiated under basal conditions. Cuf2-GFP was observed at each extremity of the cell at the end of the first meiotic division (MI) that is to say at anaphase I. Cuf2-GFP followed the segregation of chromatids until late in anaphase II when the second meiotic division (MII) occurred in early anaphase II. The Cuf2-GFP fluorescence signal was detected during the FSM formation (i.e. at the end of meiosis), but disappeared during spore formation. Cells at different stages of meiosis were stained using Hoechst 33342 to visualize the DNA (centre right). The merged images are shown in the far right panels. Nomarski optics (far left) were used to monitor cell morphology.