FIG. 1.

Stra8 and Dnmt3l expressions in germ cells cultured with RAR inhibitor. Isolated XX and XY germ cells at 11.5 and 12.5 dpc were cultured on mesh inserts with RAR inhibitor (at 0, 10⁻⁶, and 10⁻⁷ M) for 4 and 3 days, respectively. Stra8 expression levels were decreased in all germ cell groups in the presence of the RAR inhibitor (A). Dnmt3l expression was detected only in XY germ cells at 12.5 dpc (B). Gene expression levels are shown relative to those of an untreated control (which is set as 1 for each gene). Normalization of expression was achieved against the amplification of Actb. Values are means ± SEM from 3 separate experiments.