Table 3. pH, product and NADH/NAD+ ratios of different recombinant strains at initial growth pH of 7.3 after 12 h aerobic culture.
Concentrations of pyruvate metabolites (mM) | ||||||
Strains | Final pH | Lactate | Acetate | Diacetyl | Acetoin | NADH/NAD+ ratio |
DA | 5.93±0.03 | 23.41±0.11 | 8.47±0.14 | ND | ND | 0.741±0.009 |
DA/pA17nox | 6.02±0.04 | 21.15±0.08 | 8.40±0.15 | 1.07±0.03 | 0.69±0.02 | 0.711±0.005 |
DA/pA1nox | 6.05±0.02 | 20.28±0.13 | 8.43±0.09 | 1.19±0.01 | 0.87±0.05 | 0.689±0.006 |
DA/pA12nox | 6.05±0.05 | 19.86±0.29 | 9.26±0.1 | 1.51±0.03 | 0.92±0.03 | 0.681±0.002 |
DA/pA56nox | 6.07±0.02 | 19.09±0.12 | 9.12±0.14 | 1.8±0.01 | 1.01±0.04 | 0.556±0.008 |
DA/pA97nox | 6.10±0.01 | 18.15±0.21 | 10.29±0.13 | 1.8±0.04 | 1.05±0.01 | 0.55±0.005 |
DA/pA19nox | 6.10±0.01 | 17.46±0.17 | 11.08±0.07 | 1.84±0.05 | 1.05±0.03 | 0.541±0.005 |
DA/pA32nox | 6.12±0.02 | 16.24±0.1 | 11.96±0.08 | 2.02±0.04 | 1.38±0.01 | 0.462±0.002 |
DA/pD2nox | 6.14±0.04 | 14.21±0.13 | 13.11±0.12 | 2.26±0.02 | 1.67±0.05 | 0.457±0.002 |
DA/pB89nox | 6.18±0.01 | 13.00±0.09 | 13.42±0.21 | 2.39±0.06 | 1.96±0.04 | 0.413±0.004 |
DA/pB21nox | 6.22±0.02 | 11.76±0.16 | 13.09±0.01 | 3.38±0.12 | 2.14±0.04 | 0.406±0.003 |
DA/pB6nox | 6.32±0.03 | 9.94±0.07 | 12.34±0.14 | 4.16±0.06 | 2.83±0.06 | 0.383±0.003 |
ND, not detected. The values are means ± standard deviations for three independent experiments.