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. 2012 Apr 27;7(4):e36359. doi: 10.1371/journal.pone.0036359

Figure 1. Expression of ligands and E3 ligases for Notch signaling in DCs.

Figure 1

A and B, Semi-quantitative RT-PCR analyses of Notch ligands (A) and E3 ubiquitin ligases (B) in BM-derived DCs prior to LPS stimulation. Serial 2-fold dilutions of PCR products were electrophoresed in agarose gel C, Quantitative real-time RT-PCR analysis of ligands and E3 ligases in DCs before (None) and after 24 h of LPS stimulation (Sti). The results are representative of more than five independent experiments. D, Flow cytometry analysis for Notch ligands in DCs. IgG, Armenian hamster IgG isotype control; None, DCs before LPS stimulation; Sti, DCs after LPS stimulation. Results are representative of three independent experiments. E, Western blot analysis of Mib1 in BM culture with rGM-CSF on days 5, 7, and 9, and 24 h after LPS treatment on day9 (Sti). F, Quantitative real-time RT-PCR analysis of Mib1 in BM culture with rGM-CSF on days 5, 7, and 9. G, Quantitative real-time RT-PCR analysis of Mib1 in the BM precursors of classical spleen DC. MP, myeloid progenitor; MDP, macrophage and DC precursor; CDP, common DC precursor; pre-cDC, committed precursors of classical spleen DC. Results are representative of three independent experiments. Data represent mean ± SD; *, P<0.05.