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. 2012 Apr 27;7(4):e36359. doi: 10.1371/journal.pone.0036359

Figure 3. Mib1-null DCs fail to activate Notch signaling.

Figure 3

A, LPS-stimulated Mib1f/f or Mx1-Cre;Mib1f/f DCs were cultured with Notch1-expressing C2C12 cells transfected with WT or MT CBF luciferase constructs, and 24 h after the coculture, luciferase activity was measured. Results are representative of two independent experiments. B, Western blot analysis of Notch1 ICD (NICD) in negatively purified OT-II CD4+ T cells before (None) and 6 h after coculture with peptide-pretreated Mib1f/f or Mx1-Cre;Mib1f/f DCs. C, Quantitative real-time RT-PCR analysis of Notch target genes, Hes1 and Hes5, in CD4+ T cells before (None) and after coculture with Mib1f/f or Mx1-Cre;Mib1f/f DCs. Results are representative of three independent experiments. Data represent mean ± SD; *, P<0.05; **, P<0.001.