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. 2012 Apr 27;7(4):e36359. doi: 10.1371/journal.pone.0036359

Figure 5. Absence of Mib1 in DCs specifically impairs Th2 differentiation.

Figure 5

A, Purified naive OT-II CD4+ T cells were cultured with peptide pretreated Mib1f/f or Mx1-Cre;Mib1f/f DCs. After 5 days, viable cells were re-stimulated with PMA and ionomycin for 6 h, and the intracellular levels of IFN-γ and IL-4 were analyzed by flow cytometry. The numbers indicate the percentage of cells within the gates. A representative of three independent experiments is shown. B, The average percentage of activated CD4+ T cells producing IFN-γ and IL-4 (as in [A]) from three independent experiments. C, Viable activated CD4+ T cells were harvested, and an equal number of cells in each group were re-stimulated with 1 µg/ml plate-bound anti-CD3. The supernatants were removed after 48 h, and cytokine concentrations were determined by ELISA. D, Quantitative real-time RT-PCR analysis of T-bet, Gata-3, Th1 related cytokines (IFN-γ and TNF-α), and Th2 related cytokines (IL-4, IL-6, IL-10, and IL-13) in purified CD4+ T cells unstimulated (None) or stimulated by Mib1f/f or Mx1-Cre;Mib1f/f DCs. Data represent mean ± SD; *, P<0.05.