Figure 2. The measurements of quantal brightness of monomeric EGFP for two distinct sets of imaging conditions (referred as “Set I” and “Set II” throughout the text).

Set I corresponds to pixel size of 0.046 µm, with the scan speed set to “fast” (18.1 µm/µs), and Set II - pixel size of 0.0921 µm, with the scan speed set to “slow” (10.1 µm/µs). The values of ε were recovered with both fluorescence moment image analysis and SpIDA for comparison purposes. The error bars were calculated as the standard error of images of multiple cells. A) measured quantal brightness for various values of the mean intensity; B) average values (with error bars) for sets I & II.