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. 2012 Apr 27;7(4):e36215. doi: 10.1371/journal.pone.0036215

Figure 10. Dual-color CLSM images of HEK293 cells transiently expressing NBCe1-A-EGFP immunostained with Alexa Fluor 647 conjugated antibody.

Figure 10

Post fixation, cells were immunolabeled with the primary anti-wt-NBCe1-A antibody and the secondary Alexa Fluor 647 conjugated antibody. Panel A shows the fluorescence image taken in channel 0 (green), Panel B shows the image recorded in channel 1 (red), and Panel C is the overlap of the two. The image contrast was enhanced for better visualization of non-specific binding of the secondary Alexa Fluor 647 conjugated antibody to HEK293 cells. The pixel size is 0.0921 µm.