Figure 11. Non specific binding control of the antibody used for kidney tissue staining.

HEK293 cells were transiently transfected with NBCe1-A-EGFP mutant. Post fixation, cells were immunolabeled with both primary anti-wt-NBCe1-A antibody (I) and secondary Alexa Fluor 647 conjugated antibody (II) (right bar), and with secondary Alexa Fluor 647 conjugated antibody alone (left bar). The moment analysis reveals non-significant binding of both primary and secondary antibody together. The secondary antibody was identical to the one used for tissue immunostaining except it was labeled with Alexa 647.