Figure 2. Extent of tissue-specific ASM isoform expression.

A. Human tissues. ASM isoform expression varied tissue-specifically, depending on the type of splicing event. Isoform fractions derived from splicing events between exons 2 and 4 showed high levels of variation, with brain tissue expressing the highest percentage of ASM isoforms. Isoform fractions derived from splicing events between exons 4 and 6 showed low levels of variation. Capillary electrophoresis with laser-induced fluorescence analysis was conducted on triplicate RNA isolations of different human tissues. Data indicate ASM isoform percent fractions of splicing events occurring between exons 2 and 4 and exons 4 and 6. B. Human lymphoblastoid cell lines. ASM isoform expression in human lymphoblastoid cell lines revealed a low level of inter-individual variation for splicing events occurring between exons 2 and 4 and exons 4 and 6. Capillary electrophoresis with laser-induced fluorescence analysis was conducted on triplicate RNA isolations. Data indicate ASM isoform percent fractions of splicing events occurring between exons 2 and 4 and exons 4 and 6. C. Human primary blood cells. ASM isoforms were expressed in each of the 22 healthy individuals’ blood cell RNA, but the relative contribution of ASM isoforms to the total amount of ASM transcripts varied highly between subjects. Capillary electrophoresis with laser-induced fluorescence analysis was conducted. Data indicate ASM isoform percent fractions of splicing events occurring between exons 2 and 6.