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. 2012 Apr 27;7(4):e35906. doi: 10.1371/journal.pone.0035906

Figure 4. The effect of LSA on the anti-CCR5/CXCR4 mAb binding.

Figure 4

CCR5-specific mAb binding to Ghost (3) X4/Hi5 cells (A) were inhibited by LSA (dashed block) or Nifeviroc, a CCR5 antagonist serving as a control (solid block), or CXCR4-specific mAb bindings to Ghost (3) X4/Hi5 cells (B) were inhibited by LSA (dashed block) or AMD3100, a CXCR4 antagonist serving as a control (solid block). Ghost (3) X4/Hi5 cells in suspension were incubated with anti-CCR5/CXCR4 mAbs in the presence of various concentrations of LSA or Nifeviroc for 30 minutes at 4°C. The binding of the mAbs to the cell surface co-receptors was measured by FACS. Data shown were the average of three independent experiments. Error bars denote standard errors of individuals.