Table 2. Chromate reductase activity of ChrR mutant enzymes.
Enzyme | nM Cr(VI) reduced/min/mg protein | Enhancement factor |
ChrR WT | 860 (±50) | |
ChrR 21 - Tyr128Asn | 18065 (±1123)* | ×21 |
ChrR 41 - Glu146Thr | 25014 (±1007) | ×29 |
ChrR 42 - Tyr85Asn | 11496 (±134) | ×13 |
ChrR 43 - Arg125Met | 8093 (±951) | ×9,4 |
ChrR21 activity has been previously reported [7]. It was re-determined since.
protein preparation procedure and reaction mixtures used in this study are different from those used previously.