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. 2012 Apr 27;7(4):e35826. doi: 10.1371/journal.pone.0035826

Figure 2. FGF2 activates WNT/β-catenin signaling via LRP6 phosphorylation.

Figure 2

(A) FGF2-treated RCS cells were analyzed by WB for LRP6 phosphorylation at Ser1490 (signal quantified by densitometry) or Thr1572. Total LRP6 and ACTIN serve as loading controls. C1, C2 - untreated cells. (B) Schematic representation of the LRP6 expression vectors: intracellular PPPS/TP motifs are indicated (1–5), including the position of Ser1490 (motif 1) or Thr1572 (motif 3) recognized by the antibodies used in (A). M - cell membrane. Asterisks indicate Ser/Thr in the PPPS/TP motifs that were replaced by Ala. (C, D) Cells were transfected with LRP6 or empty vector together with Topflash reporter vectors, treated as indicated, and analyzed for luciferase activity. Data represent an average from four transfections (each measured twice), with the indicated standard deviations (* p<0.001; Student’s t-test). Results are representative of three experiments.