Figure 9. Unfractionated NOMID bone marrow cells proliferate and survive significantly less than their WT counterparts.

Unfractionated bone marrow cells (A–C) or BMM (D and E) were cultured in media containing M-CSF for the indicated times. Proliferation (A and D), metabolic activity (B and E) and Western blot analysis of PARP cleavage (C) were carried out. While unfractionated NOMID cells proliferated and survived less than WT cells, no differences were seen in BMM proliferation and survival between genotypes. PARP cleavage was higher in NOMID than in WT cells. Determinations were performed in triplicate and expressed as the mean ± S.D. Results are representative of three independent experiments. *P<0.05; **P<0.007 over the control (Ctl).