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. 2012 Apr 27;7(4):e35855. doi: 10.1371/journal.pone.0035855

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Thomas et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The solubility of PfGAP45-GFP or its mutants and endogenous PfGAP45 was assessed by performing extractions in PBS, PBS/1 M NaCl, PBS/0.1 M Na₂CO₃ pH 11.0 or PBS/0.1% Triton ×100. The supernatant (S) and the pellet (P) fractions were subjected to western blotting using anti GFP and anti GAP45 antibody (bottom panel) as indicated.