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. 2012 Apr 25;7(4):e35053. doi: 10.1371/journal.pone.0035053

Figure 3. Physical structure of φB124-14 genome. Left and middle panels.

Figure 3

show in silico digest and electrophoresis to visualise restriction fragment profiles of φB124-14 expected for each permutation of the genome (linear or circular) generated by pDRAW32. Right panel shows results obtained from digestion of 1.5 µg of φB124-14 DNA (3 h at 37°C) with restriction enzymes used in in silico analysis. Restriction enzymes tested are indicated above each lane. MW  = 1 kb Molecular Weight marker (Promega). UC  =  uncut φB124-14 DNA.