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. 2012 Apr 25;7(4):e34925. doi: 10.1371/journal.pone.0034925

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Durward et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A) Representative flow cytometric gating strategy of splenic T_mem, CD8+ LFA1^hiCD127^hiKLRG1^lo at day 60 post-infection. From left to right the panels show gating of CD8+ cells, LFA1^hiCD8+ cells, and CD8+ LFA1^hiCD127^hiKLRG1^lo cells from analysis of 500,000 to 1 million cells. B) Plots showing the absence of T_mem in age-matched uninfected mice (PBS injected) and the presence of T_mem in mice with chronic brucellosis. C) Frequency of splenic CD8+ T_mem over time. ANOVA was performed to determine if infection with B. melitensis had a statistically significant impact on the presence of CD8+ Tmem. **p<0.002 for infected animals at the different days except for day 150 where p<0.05.