Figure 5. Cytokine co-expression profile of CD8+ T cells expressing 3 or 2 cytokines simultaneously.

A) Frequency of polyfunctional splenic CD8+ T cells co-expressing IFN-γ, TNF-α, and IL-2. Expression of 3 cytokines from infected animals was not different from PBS animals over the time of infection, p≤0.06 at 180 days. B) Frequency of dual-function CD8+ T cells co-expressing IFN-γ and TNF-α, p≤0.7 on any day. C) Frequency of dual-function CD8+ T cells co-expressing IFN-γ and IL-2, p≤0.8 on any day.