Figure 3. CPDT and SF block Nrf2 degradation mainly in the nucleus, but do not dissociate the Nrf2-Keap1 complex or the ubiquitination complex.

RT-4 cells and NBT-II cells were treated with vehicle, CPDT (50 µM) or SF (8 µM) for 6 h, from which cytosols and nuclear extracts were prepared and were subjected to analysis by IB or IP followed by IB. The loading amount of the nuclear sample was about half of the cytoplasmic sample for both IP and IB. (A) IB of indicated proteins. GAPDH and lamin A were used to confirm the purity of the cytosols and nuclear extracts, respectively. (B) Cytosols and nuclear extracts were subjected to IP with anti-Keap1, followed by IB of the indicated proteins.