Table 1.

Optimized Multiplicity of Infections (MOIs) and Incubation Times for Cytokines Specific to Measles Vaccine Virus

Cytokine	MOI	Incubation time (hours)
IL-2	0.5	48
IL-6	1.0	72
IL-10	0.5	48
IFN-α	1.0	24
IFN-γ	1.0	72
IFN-λ1	1.0	72
TNF-α	1.0	24

ELISA assays were performed to measure the level of secreted cytokines (IL-2, IL-6, IL-10, IFN-α, IFN-γ, IFN-λ1, and TNF-α) after in vitro stimulation with measles virus using pre-optimized conditions for culture time and virus MOI, as previously described [23].