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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Anal Biochem. 2012 Feb 28;425(1):21–27. doi: 10.1016/j.ab.2012.02.027

Figure 2.

Figure 2

Fluorescence vs. [ThioGlo-ACP] plot. The fluorescence of various concentrations of purified ThioGlo-ACP conjugate, in 20 mM HEPES pH 8 (total volume 100 μL), was determined at 25 °C using a SpectraMax M5 plate reader.