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Saturation kinetics of LpxA for A) UDP-GlcNAc and B) R-3-hydroxymyristoyl-ACP. A) UDP-GlcNAc was varied between 150–4000 μM, while holding acyl-ACP constant at 40 μM. B) R-3-hydroxymyristoyl-ACP was varied over a concentration range of 1–40 μM, while holding UDP-GlcNAc constant at 4000 μM. Assays were run in triplicate.
