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Saturation kinetics of LpxD for A) UDP-3-O-(R-3-hydroxymyristoyl)-GlcN and B) R-3-hydroxymyristoyl-ACP. A) UDP-3-O-(R-3-hydroxymyristoyl)-GlcN was varied over a range of 0.35–19 μM while holding acyl-ACP constant at 40 μM. B) acyl-ACP was varied over a concentration range of 1–40 μM while holding UDP-3-O-(R-3-hydroxymyristoyl)-GlcN constant at 19 μM. Assays were run in triplicate.
