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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Lab Invest. 2012 Feb 13;92(5):703–712. doi: 10.1038/labinvest.2012.3

Fig. 5. Effect of HB-EGF on fibrogenic gene expression in HSC primary culture.

Fig. 5

Stellate cells isolated from untreated healthy Balb/c wild type mice (A) or B6;129-Hbegftm1Dcl/Mmnc HB-EGF WT mice (B, C) were cultured for 3 days. Cells were pre-incubated in DMEM/F-12 containing 0.5% fetal bovine serum for 24 hours, followed by treatment with recombinant human HB-EGF for an additional 24 hours with (C) or without (A, B) 10 µg/ml CRM197 prior to analysis of mRNA expression of α-SMA, COL1A1 or CCN2 by quantitative real-time PCR. Data are the mean ± S.D. of three experiments with triplicate determinations. *p < 0.05 vs control (Ctrl); **p < 0.05 vs HB-EGF.