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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Feb 23;1822(6):952–960. doi: 10.1016/j.bbadis.2012.02.015

Figure 3.

Figure 3

Effect of TF on insulin-stimulated Akt phosphorylation in human adipocytes. Human adipose tissue was pretreated for 48 hr with 100 nM TF, and then insulin dose response curves were performed. A representative immunoblot of insulin-stimulated Akt phosphorylation is shown (Panel A). The phosphorylated-to-total Akt ratio was used to determine insulin action in human fat obtained from surgical patients (n = 4 except for 5 nM insulin where n = 3) (Panel B). Data were normalized to vehicle-treated adipocytes exposed to 10 nM insulin. * p < 0.05.