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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Feb 23;1822(6):952–960. doi: 10.1016/j.bbadis.2012.02.015

Figure 6.

Figure 6

Time- and Concentration-Dependence of Tolyfluanid-Mediated IRS-1 Downregulation. For time-dependence (Panel A), RNA was isolated from cultured C57BL/6 perigonadal fat pads after 12–48 h incubation with 100 nM tolylfluanid or vehicle (ethanol, EtOH). For concentration-dependence (Panel B), RNA was similarly isolated after exposure to vehicle or TF at concentrations ranging from 100 pM to 1μM. Isolated RNA was reverse transcribed, and qRT-PCR was used to assess fold change differences between vehicle- versus TF-treated adipocytes. Data are presented as means ± S.E.M. of the fold induction of IRS-1 relative to 18S rRNA normalized to vehicle-treated controls (n = 3–5 for time-dependence; n = 4 for concentration-dependence). * p < 0.05; ** p < 0.01; *** p < 0.001.