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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Feb 23;1822(6):952–960. doi: 10.1016/j.bbadis.2012.02.015

Figure 7.

Figure 7

Effect of TF on IRS-1 Protein Stability. Perigonadal fat pads from C57BL/6 mice were treated with vehicle or 100 nM TF for 12 h followed by incubation with the protein translation inhibitor puromycin at a final concentration of 100 μM for an additional 2 h. Levels of Akt were measured in cell lysates, while IRS-1 protein levels were determined after immunoprecipitation. Representative immunoblots (Bottom) are shown as well as cumulative densitometry for IRS-1 as means ± S.E.M. normalized to vehicle-treated controls (n = 3). IRS-1 densitometry is expressed as IRS-1/Akt normalized to vehicle (ethanol, EtOH). * p < 0.05; *** p < 0.001.