Table 1.

Secondary structure changes and photorecovery times

Construct	δ222a	FMN τ (s)
WT	0.30	81
WT(404–546) W491Y/L408W	0.30	78
ΔJα	0.15	82
ΔJα W491Y/L408W	0.16	79
Δ408–411 ΔJα	0.10	83
T406A/T407A	0.38	56
T407P	0.27	81
T407W	0.29	82
Δ408–411	0.11	71
L408D	0.18	96
R410P	0.20	108
R410G	0.35	78
Δ410–412	0.34	72
K413A	0.22	59
N414V	0.25	≥ 12 hrsb
F434L	0.35	12
E475A	0.20	67
Q479L	0.30	49
N492A	0.18	54
L493A	0.11	121
F494L	0.15	206
H495L	0.15	85
Q497A	0.38	74
R500A	0.39	57
Q502A	0.30	78
Q513A	0.18	261
L514A	0.42	83
D515V	0.25	54
H519A	0.30	80
E412H/Q436H	0.27	39

^aδ₂₂₂ = (θ_222,lit − θ_222,dark)/θ_222,dark

^bAggregation prone.