Fig. 3.

Effect of anti-Amot vaccination on the growth of transplantable TUBO tumors. BALB/c mice were challenged with TUBO cells and electroporated with pAmot or pcDNA3 both when the TUBO tumor reached 4 mm mean diameter and 7 days later. a Progression of TUBO tumors: each line depicts the growth of a single tumor in pAmot vaccinated mice (n = 29). Grey area: tumors growing in the controls electroporated with pcDNA3 plasmid (n = 28). Data were cumulated from three independent and concordant experiments. b Titer of antibodies against Amot (white bars) and against neu (black bars) in the sera of fast and low progressor mice electroporated with pAmot and in controls electroporated with the pcDNA3 plasmid. The titers of anti-Amot antibodies are significantly higher in slow progressor mice, P = 0.03 (Student’s t test)