Figure 7.
MRM chromatograms recorded for three selected HCPs measured in Sample B1. Each part displays the best responding transitions for the native and the corresponding 13C15N-isotopically labeled peptide belonging to (A and D) clusterin, (B and E) elongation factor 1α and (C and F) glyceraldehyde 3-phosphate dehydrogenase. MRMs displayed in (A–C) were recorded using the analytical scale chromatography setup (2.1 mm column ID), and the chromatograms presented in (D–F) were obtained on the prototype TRIZAIC nanoTile microfluidic device (150 µm channel ID).