Figure 4.
The progress of amyloid fibril formation measured by fluorimetric Thioflavin T uptake assay in presence of seeds. One percent (w/w) performed fibrillar seeds (denoted in square brackets) were added to solutions containing monomers of sheep (A) or deer (B) Y145Stop. No seed control showed the characteristic lag phase, while seeded reactions with partially purified PrPSc from deer [CWD] or sheep [Sc] eliminated the lag phase. Similar conversion rates were observed with cross seeding by in-vitro converted Y145Stop of sheep [ShY145Stop] or deer [DeY145Stop].