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. 2012 Jan 1;6(1):81–88. doi: 10.4161/pri.6.1.18493

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Copyright © 2012 Landes Bioscience

PMC Copyright notice

PMCA on purified PrP^C from brain tissue of sheep and deer. Lane 1, molecular weight marker (biotinylated). Lane 2, human recombinant PrP 23–231 was used as a positive control. Lanes 3 and 4, samples containing purified PrP^C from brain tissue of sheep before and after PK digestion. Lane 5, PrP^Sc extracted from sheep brain as a reference after PK digestion with 10 times the reaction equivalents of the seed protein concntration. Samples of purified PrP^C from brain tissue of sheep were incubated with preformed fibrillar seed of PrP^Sc round one (lane 6), round two (lane 7), PrP^CWD (lane 8), ShY145Stop seeded with performed fibrils of ShY145Stop (lane 10), DeY145Stop (lane 11), or PrP^Sc (lane 12) after PK digestion.