Figure 1.

Schematic summary of the differentiation conditions used in the generation of dopaminergic neurons (a) Schematic diagram illustrating the different stages of NPC generation and dopaminergic neuronal differentiation. The abbreviations are. Dor/SB: Dorsomorphin and SB431542, EB: EB media, NIM: neural induction media, NPC: neural progenitor cell media, and DA1/DA2: medium for dopaminergic differentiation. (b) Timeline shows the medium used at different stages of NPCs and dopaminergic maturation and displays the sampling dates for performing gene expression studies, such as qPCR, marker characterization (immunofluorescence: IF), to analyze efficiency of rosette formation and for quantitative studies of TUJ1 and TH immunoreactivity using stereology and flow cytometry.