Skip to main content
. 2012 Apr 10;2012:140427. doi: 10.1155/2012/140427

Figure 3.

Figure 3

Representative images of neural rosettes at different stages (A–D). Neural rosettes after Dor/SB treatment at days 7–12, stage III of the differentiation protocol (5x magnification). Arrows indicate boundary lines in the early and late stages of rosettes. (E) and (F) Manual dissection of rosettes using scalpel are illustrated before (E) and after (F) cutting. (G and H) Arrows indicate the positions of neural rosettes that were immunoreactive with Pax6 (green) (E) and Sox1 (red) (F). Scale bar represents 20 μm. (i) There is a significant difference in neural rosette formation in all control and PD-specific EBs treated with Dor/SM compared to those with no Dor/SB treatment during stage II (*P < 0.01). Data are presented as mean + standard error of the mean (SEM) compared to the controls (n = 3, except 1679, n = 2). P value of each study was assessed by one-way ANOVA along with Newman-Keuls post-hoc analysis.