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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Neurobiol Dis. 2011 Sep 3;45(1):381–387. doi: 10.1016/j.nbd.2011.08.027

Fig. 1.

Fig. 1

Flow cytometry analysis of VGluT1 and VGluT2 in control and AD synaptosomes. Human cortical synaptosomal preparation immunolabeled with (A) non-immune IgG and (B) SNAP-25. Positive control sample labeled for SNAP-25 with side scatter dot plot showing polystyrene size standards (C). Synaptosome integrity assessed with the fluorescent dye, Fluo-4NW (D). Representative density plots of normal (E and F) and AD (G and H) synaptosomes labeled for VGluT1 (E-G) and VGluT2 (F-H). The forward scatter parameter on the X-axis is proportional to size. Synaptic distribution of VGluT1 and VGluT2 in control and AD parietal cortex are shown (I). Mixed model ANOVA, VGluT * group interaction [F =66.90; P < 0.001], values represent means ± SEM. *, P < 0.05 between VGluT1-IR in AD compared to controls.