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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Neurobiol Dis. 2011 Sep 3;45(1):381–387. doi: 10.1016/j.nbd.2011.08.027

Fig. 4.

Fig. 4

Co-localization of VGluT1-2 and Aβ. (A) Quadrant analysis of human cortical synaptosomal preparation immunolabeled with non-immune IgG. A representative sample of AD parietal cortex double labeled for VGluT1 and Aβ (B) and VGluT2 and Aβ (C).Quadrant analysis for one representative aged normal control sample double labeled for VGluT1 and Aβ (D) and VGluT2 and Aβ (E). Glutamatergic synaptosomes containing Aβ (Abeta+/VGluT+) are in the upper right quadrant, non-glutamatergic synaptosomes positive for Aβ (Abeta+/VGluT−) are in the lower right quadrant and glutamatergic synaptosomes lacking Aβ (Abeta−/VGluT+) are in the upper left quadrant. Numbers represent the percentage of synaptosomes in each quadrant. (F) Aβ variation in single-labeled (Abeta+/VGluT− only, silver bars; Abeta−/VGluT+ only, grey bars) and dual-labeled (Abeta+/VGluT+, black bars) fractions of six AD parietal cortices. Due to the low Aβ levels in aged normal controls, this analysis was only performed in AD cases. Mixed model ANOVA, VGluT * Aβ interaction [F = 7.27; P < 0.01]. Values represent means ± SEM. *P<0.05 and **P < 0.01, dual-labeled compared to single-labeled fractions; ##P < 0.0001 Abeta+/VGluT1+ dual-labeled fractions compared to Abeta+/VGluT2+ dual-labeled fractions.